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rabbit anti cd86 antibody  (Bioss)
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Bioss rabbit anti cd86 antibody
Rabbit Anti Cd86 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti cd86 polyclonal antibody  (Elabscience Biotechnology)
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rabbit polyclonal anti cd86 antibody  (Servicebio Inc)
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Immunohistochemical detection of CD163 + and <t>CD86</t> + TAMs in colorectal tissues (×200). (A) CD163 staining in various tissues. (B) CD86 staining. (C) IOD for CD163. (D) IOD for CD86. Data expressed as M (Q1, Q3) (n = 109). * P < 0.05, ** P < 0.01, *** P < 0.001 (DB-adjusted). Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. M: Median; Q 1 : 1st Quartile; Q 3 : 3rd Quartile.
Rabbit Polyclonal Anti Cd86 Antibody, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd86 rabbit pab  (Bioss)
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Immunohistochemical detection of CD163 + and <t>CD86</t> + TAMs in colorectal tissues (×200). (A) CD163 staining in various tissues. (B) CD86 staining. (C) IOD for CD163. (D) IOD for CD86. Data expressed as M (Q1, Q3) (n = 109). * P < 0.05, ** P < 0.01, *** P < 0.001 (DB-adjusted). Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. M: Median; Q 1 : 1st Quartile; Q 3 : 3rd Quartile.
Cd86 Rabbit Pab, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd86 rabbit pab - by Bioz Stars, 2026-06
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rabbit anti cd86 polyclonal antibody  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit anti cd86 polyclonal antibody
Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and <t>CD86</t> (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.
Rabbit Anti Cd86 Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti cd86 polyclonal antibody/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
rabbit anti cd86 polyclonal antibody - by Bioz Stars, 2026-06
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  Buy from Supplier
rabbit anti cd86  (Bioss)
95
Bioss rabbit anti cd86
Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and <t>CD86</t> (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.
Rabbit Anti Cd86, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti cd86/product/Bioss
Average 95 stars, based on 1 article reviews
rabbit anti cd86 - by Bioz Stars, 2026-06
95/100 stars
  Buy from Supplier
rabbit anti cd86 polyclonal antibody  (Bioss)
95
Bioss rabbit anti cd86 polyclonal antibody
Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and <t>CD86</t> (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.
Rabbit Anti Cd86 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti cd86 polyclonal antibody/product/Bioss
Average 95 stars, based on 1 article reviews
rabbit anti cd86 polyclonal antibody - by Bioz Stars, 2026-06
95/100 stars
  Buy from Supplier

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Immunohistochemical detection of CD163 + and CD86 + TAMs in colorectal tissues (×200). (A) CD163 staining in various tissues. (B) CD86 staining. (C) IOD for CD163. (D) IOD for CD86. Data expressed as M (Q1, Q3) (n = 109). * P < 0.05, ** P < 0.01, *** P < 0.001 (DB-adjusted). Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. M: Median; Q 1 : 1st Quartile; Q 3 : 3rd Quartile.

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Immunohistochemical detection of CD163 + and CD86 + TAMs in colorectal tissues (×200). (A) CD163 staining in various tissues. (B) CD86 staining. (C) IOD for CD163. (D) IOD for CD86. Data expressed as M (Q1, Q3) (n = 109). * P < 0.05, ** P < 0.01, *** P < 0.001 (DB-adjusted). Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. M: Median; Q 1 : 1st Quartile; Q 3 : 3rd Quartile.

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Immunohistochemical staining, Staining

Correlation of CD163 + /CD86 + TAMs levels with CRC development. (A) Differential expression between H.pylori -infected and uninfected groups. (B) Correlation between CD163 + and CD86 + expression. (C, D) Positive correlation of both markers with malignancy grade. Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. *** P < 0.001 (MWU test).

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Correlation of CD163 + /CD86 + TAMs levels with CRC development. (A) Differential expression between H.pylori -infected and uninfected groups. (B) Correlation between CD163 + and CD86 + expression. (C, D) Positive correlation of both markers with malignancy grade. Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. *** P < 0.001 (MWU test).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Quantitative Proteomics, Infection, Expressing

Multiplex immunofluorescence (200×) : CD68 + (red), CD163 + (green), CD86 + (green), DAPI (nuclei, blue), Merge (multichannel overlay), Scale bar: 50 μm. (A) CD68 + CD163 + dual-positive cells (IF). (B) CD68 + CD86 + dual-positive cells (IF).

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Multiplex immunofluorescence (200×) : CD68 + (red), CD163 + (green), CD86 + (green), DAPI (nuclei, blue), Merge (multichannel overlay), Scale bar: 50 μm. (A) CD68 + CD163 + dual-positive cells (IF). (B) CD68 + CD86 + dual-positive cells (IF).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Multiplex Assay, Immunofluorescence

Changes in cell density and proportion of CD68 + CD163 +/ CD68 + CD86 + dual-positive TAMs across groups. Groups: 1, Normal; 2, CRA; 3, CRC. *** P < 0.001 (Tukey HSD).

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Changes in cell density and proportion of CD68 + CD163 +/ CD68 + CD86 + dual-positive TAMs across groups. Groups: 1, Normal; 2, CRA; 3, CRC. *** P < 0.001 (Tukey HSD).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques:

Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and CD86 (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.

Journal: Cardiovascular Diagnosis and Therapy

Article Title: Establishment of a medial arterial calcification model in C57BL/6J mice via arterial intimal injury

doi: 10.21037/cdt-2025-435

Figure Lengend Snippet: Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and CD86 (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.

Article Snippet: Subsequently, samples were incubated with rabbit anti-CD86 polyclonal antibody (1:100; Cat #19589; Cell Signaling Technology, Danvers, USA) diluted in 1% BSA/PBS at 4 °C overnight.

Techniques: Staining, Expressing, Fluorescence, Immunohistochemistry